A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA

Nguyen, Kim; Kubota, Miles; Arco Arrieta, Jon del; Feng, Chao; Singha, Monika; Beasley, Samantha; Sakr, Jasmine; Gandhi, Sunil P.; Blurton-Jones, Matthew; Spitale, Robert C.; Et al.

doi:10.1021/acschembio.0c00755

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A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA

Nguyen, Kim; Kubota, Miles; Arco Arrieta, Jon del; Feng, Chao; Singha, Monika; Beasley, Samantha; Sakr, Jasmine; Gandhi, Sunil P.; Blurton-Jones, Matthew; Spitale, Robert C.; Et al.

Use this identifier to cite or link to this item: http://hdl.handle.net/11268/10954

Date: 2020

UNESCO Subject: Bioquímica
UNESCO Subject: Biología celular

Type: article

Editor's version: https://doi.org/10.1021/acschembio.0c00755

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Abstract:

Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.

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