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New insights on nucleoside 2'-deoxyribosyltransferases: a versatile biocatalyst for one-pot one-step synthesis of nucleoside analogs

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dc.contributor.author Fresco-Taboada, Alba spa
dc.contributor.author Mata Riesco, Isabel de la spa
dc.contributor.author Arroyo Sánchez, Miguel spa
dc.contributor.author Fernández Lucas, Jesús spa
dc.date.accessioned 2014-03-18T10:09:19Z
dc.date.available 2014-03-18T10:09:19Z
dc.date.issued 2013 spa
dc.identifier.citation Fresco-Taboada, A., Mata-Riesco, I., Arroyo-Sánchez, M., & Fernández-Lucas, J. (2013). New insights on nucleoside 2′-deoxyribosyltransferases: a versatile biocatalyst for one-pot one-step synthesis of nucleoside analogs. Applied Microbiology and Biotechnology, 97(9), 3773-3785. spa
dc.identifier.uri http://hdl.handle.net/11268/2198
dc.description.abstract In recent years, glycosiltransferases have arisen as standard biocatalysts for the enzymatic synthesis of a wide variety of natural and non-natural nucleosides. Such enzymatic synthesis of nucleoside analogs catalyzed by nucleoside phosphorylases and 2'-deoxyribosyltransferases (NDTs) has demonstrated to be an efficient alternative to the traditional multistep chemical methods, since chemical glycosylation reactions include several protection-deprotection steps. This minireview exhaustively covers literature reports on this topic with the final aim of presenting NDTs as an efficient option to nucleoside phosphorylases for the synthesis of natural and non-natural nucleosides. Detailed comments about structure and catalytic mechanism of described NDTs, as well as their possible biological role, substrate specificity, and advances in detection of new enzyme specificities towards different non-natural nucleoside synthesis are included. In addition, optimization of enzymatic transglycosylation reactions and their application in the synthesis of natural and non-natural nucleosides have been described. Finally, immobilization of NDTs is shown as a practical procedure which leads to the preparation of very interesting biocatalysts applicable to industrial nucleoside synthesis. spa
dc.language.iso eng spa
dc.title New insights on nucleoside 2'-deoxyribosyltransferases: a versatile biocatalyst for one-pot one-step synthesis of nucleoside analogs spa
dc.type article spa
dc.description.impact 3.811 JCR (2013) Q1, 32/165 Biotechnology & applied microbiology spa
dc.identifier.doi 10.1007/s00253-013-4816-y spa
dc.rights.accessRights closedAccess en
dc.subject.unesco Tecnología química spa
dc.subject.unesco Química spa
dc.subject.unesco Biotecnología spa
dc.peerreviewed Si spa


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