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Nucleoside 2'-Deoxyribosyltransferase from psychrophilic bacterium bacillus psychrosaccharolyticus - Preparation of an immobilized biocatalyst for the enzymatic synthesis of therapeutic nucleosides

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dc.contributor.author Fresco-Taboada, Alba
dc.contributor.author Serra, Immacolata
dc.contributor.author Fernández Lucas, Jesús
dc.contributor.author Acebal Sarabia, Carmen
dc.contributor.author Arroyo Sánchez, Miguel
dc.contributor.author Terreni, Marco
dc.contributor.author Mata Riesco, Isabel de la
dc.date.accessioned 2015-04-27T09:26:21Z
dc.date.available 2015-04-27T09:26:21Z
dc.date.issued 2014
dc.identifier.citation Fresco-Taboada, A., Serra, I., Fernández-Lucas, J., Acebal-Sarabia, C., Arroyo-Sánchez, M., Terreni, M., & Mata-Riesco, I. (2014). Nucleoside 2'-deoxyribosyltransferase from psychrophilic bacterium Bacillus psychrosaccharolyticus—Preparation of an immobilized biocatalyst for the enzymatic synthesis of therapeutic nucleosides. Molecules, 19(8), 11231-11249. spa
dc.identifier.issn 14203049 spa
dc.identifier.uri http://hdl.handle.net/11268/3925
dc.description.abstract Nucleoside 2'-deoxyribosyltransferase (NDT) from the psychrophilic bacterium Bacillus psychrosaccharolyticus CECT 4074 has been cloned and produced for the first time. A preliminary characterization of the recombinant protein indicates that the enzyme is an NDT type II since it catalyzes the transfer of 2'-deoxyribose between purines and pyrimidines. The enzyme (BpNDT) displays a high activity and stability in a broad range of pH and temperature. In addition, different approaches for the immobilization of BpNDT onto several supports have been studied in order to prepare a suitable biocatalyst for the one-step industrial enzymatic synthesis of different therapeutic nucleosides. Best results were obtained by adsorbing the enzyme on PEI-functionalized agarose and subsequent cross-linking with aldehyde-dextran (20 kDa and 70% oxidation degree). The immobilized enzyme could be recycled for at least 30 consecutive cycles in the synthesis of 2'-deoxyadenosine from 2'-deoxyuridine and adenine at 37 °C and pH 8.0, with a 25% loss of activity. High conversion yield of trifluridine (64.4%) was achieved in 2 h when 20 mM of 2'-deoxyuridine and 10 mM 5-trifluorothymine were employed in the transglycosylation reaction catalyzed by immobilized BpNDT at 37 °C and pH 7.5. spa
dc.language.iso eng spa
dc.rights Atribución 3.0 España *
dc.rights.uri http://creativecommons.org/licenses/by/3.0/ *
dc.title Nucleoside 2'-Deoxyribosyltransferase from psychrophilic bacterium bacillus psychrosaccharolyticus - Preparation of an immobilized biocatalyst for the enzymatic synthesis of therapeutic nucleosides spa
dc.type article spa
dc.description.impact 2.416 JCR (2014) Q2, 21/57 Chemistry, organic spa
dc.identifier.doi 10.3390/molecules190811231
dc.rights.accessRights openAccess spa
dc.subject.uem Biotecnología spa
dc.subject.unesco Biotecnología spa
dc.description.filiation UEM spa
dc.peerreviewed Si spa


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Atribución 3.0 España Except where otherwise noted, this item's license is described as Atribución 3.0 España