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NO plays a role in LPS-induced decreases in circulating IGF-I and IGFBP-3 and their gene expression in the liver 

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dc.contributor.author Priego, Teresa
dc.contributor.author Ibáñez de Cáceres, Inmaculada
dc.contributor.author Martín, Ana Isabel
dc.contributor.author Villanúa, María de los Ángeles
dc.contributor.author López-Calderón, Asunción
dc.date.accessioned 2016-08-22T09:01:34Z
dc.date.available 2016-08-22T09:01:34Z
dc.date.issued 2004
dc.identifier.citation Priego, T., Ibañez de Cáceres, I., Martín Velasco, A. I., Villanúa, M. A., & López-Calderón, A. (2004). NO plays a role in LPS-induced decreases in circulating IGF-I and IGFBP-3 and their gene expression in the liver. American Journal of Physiology - Endocrinology and Metabolism, 286(1), E50-E56. spa
dc.identifier.issn 01931849
dc.identifier.uri http://hdl.handle.net/11268/5650
dc.description.abstract In this study, we administered aminoguanidine, a relatively selective inducible nitric oxide synthase (iNOS) inhibitor, to study the role of nitric oxide (NO) in LPS-induced decrease in IGF-1 and IGFBP-3. Adult male Wistar rats were injected intraperitoneally with LPS (100 μg/kg), aminoguanidine (100 μg/kg), LPS plus aminoguanidine, or saline. Rats were injected at 1730 and 0830 the next day and killed 4 h after the last injection. LPS administration induced an increase in serum concentrations of nitrite/nitrate (P < 0.01) and a decrease in serum concentrations of growth hormone (GH; P < 0.05) and IGF-I (P < 0.01) as well as in liver IGF-I mRNA levels (P < 0.05). The LPS-induced decrease in serum concentrations of IGF-I and liver IGF-I gene expression seems to be secondary to iNOS activation, since aminoguanidine administration prevented the effect of LPS on circulating IGF-I and its gene expression in the liver. In contrast, LPS-induced decrease in serum GH was not prevented by aminoguanidine administration. LPS injection decreased IGFBP-3 circulating levels (P < 0.05) and its hepatic gene expression (P < 0.01), but endotoxin did not modify the serum IGFBP-3 proteolysis rate. Aminoguanidine administration blocked the inhibitory effect of LPS on both IGFBP-3 serum levels and its hepatic mRNA levels. When aminoguanidine was administered alone, IGFBP-3 serum levels were increased (P < 0.05), whereas its hepatic mRNA levels were decreased. This contrast can be explained by the decrease (P < 0.05) in serum proteolysis of this binding protein caused by aminoguanidine. These data suggest that iNOS plays an important role in LPS-induced decrease in circulating IGF-I and IGFBP-3 by reducing IGF-I and IGFBP-3 gene expression in the liver. spa
dc.description.sponsorship SIN FINANCIACIÓN spa
dc.language.iso eng spa
dc.title NO plays a role in LPS-induced decreases in circulating IGF-I and IGFBP-3 and their gene expression in the liver  spa
dc.type article spa
dc.description.impact 4.431 JCR (2004) Q1, 6/74 Physiology, 16/87 Endocrinology & metabolism spa
dc.identifier.doi 10.1152/ajpendo.00149.2003 spa
dc.rights.accessRights closedAccess spa
dc.subject.uem Fisiología humana spa
dc.subject.uem Endocrinología spa
dc.subject.uem Metabolismo - Enfermedades spa
dc.subject.unesco Fisiología spa
dc.subject.unesco Sistema endocrino spa
dc.subject.unesco Enfermedad nutricional spa
dc.description.filiation UEM spa
dc.peerreviewed Si spa


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