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Laminar and cellular distribution of AMPA, kainate, and NMDA receptor subunits in monkey sensory-motor cortex

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dc.contributor.author Múñoz, Alberto Eduardo
dc.contributor.author Woods, Timothy M.
dc.contributor.author Jones, Edward G.
dc.date.accessioned 2016-11-25T19:25:31Z
dc.date.available 2016-11-25T19:25:31Z
dc.date.issued 1999
dc.identifier.citation Muñoz, A., Woods, T. M., y Jones, E. G. (1999). Laminar and cellular distribution of AMPA, kainate, and NMDA receptor subunits in monkey sensory–motor cortex. Journal of Comparative Neurology, 407(4), 472-490. spa
dc.identifier.issn 00219967
dc.identifier.uri http://hdl.handle.net/11268/6004
dc.description.abstract In situ hybridization histochemistry and immunocytochemistry were used to examine lamina- and cell-specific expression of glutamate receptor (GluR) mRNAs and polypeptide subunits in motor and somatosensory cortex of macaque monkeys. Radioactive complementary RNA (cRNA) probes were prepared from cDNAs specific for α-amino-3-hydroxy-5-methylisoxozolepropionate (AMPA)/kainate (GluR1-GluR4), kainate (GluR5-GluR7), and N-methylD-aspartate (NMDA; NR1, NR2A-NR2D) receptor subunits. AMPA/kainate and NR1, NR2A, and NR2B receptor transcripts show higher expression than other transcripts. All transcripts show lamina-specific patterns of distribution. GluR2 and GluR4 mRNAs show higher expression than do GluR1 and GluR3 mRNAs. GluR6 transcript expression is higher than that of GluR5 and GluR7. NR1 mRNA expression is much higher than that of NR2 mRNAs. NR2C subunit expression is very low except for a very distinct band of high expression in layer IV of area 3b. Immunocytochemistry, using subunit-specific antisera and double labeling for calbindin, parvalbumin, or α type II Ca2+/calmodulin-dependent protein kinase (CaMKII-α), allowed identification of cell types expressing different subunit genes. GluR1 and GluR5/6/7 immunoreactivity is found in both pyramidal cells and gamma-amino butyric acid (GABA) cells; GluR2/3 immunoreactivity is preferentially found in pyramidal cells, whereas GluR4 immunoreactivity is largely restricted to GABA cells; NMDA receptor subunit immunoreactivity is far greater in excitatory cells than in GABA cells. The density of expression of AMPA/kainate, kainate, and NMDA receptor subunit mRNAs differed within and across the architectonic fields of sensory-motor cortex. This finding and the lamina- and cell-specific patterns of expression suggest assembly of functional receptors from different arrangements of available subunits in specific neuronal populations. spa
dc.description.sponsorship SIN FINANCIACIÓN spa
dc.language.iso eng spa
dc.subject.other Zoología spa
dc.title Laminar and cellular distribution of AMPA, kainate, and NMDA receptor subunits in monkey sensory-motor cortex spa
dc.type article spa
dc.description.impact 3.764 JCR (1999) Q1, 34/201 Neurosciences, 2/107 Zoology spa
dc.identifier.doi 10.1002/(SICI)1096-9861(19990517)407:4<472::AID-CNE2>3.0.CO;2-2
dc.rights.accessRights closedAccess spa
dc.subject.uem Neurociencia spa
dc.subject.uem Fisiología spa
dc.subject.unesco Neurología spa
dc.subject.unesco Fisiología spa
dc.description.filiation UEM spa
dc.peerreviewed Si spa


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