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High levels of intratumor heterogeneity characterize the expression of epithelial-mesenchymal transition markers in high-grade clear cell renal cell carcinoma

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dc.contributor.author Guarch, Rosa
dc.contributor.author Lawrie, Charles H.
dc.contributor.author Larrinaga, Gorka
dc.contributor.author Angulo Cuesta, Javier
dc.contributor.author Pulido, Rafael
dc.contributor.author López, José Ignacio
dc.date.accessioned 2018-08-13T12:28:06Z
dc.date.available 2018-08-13T12:28:06Z
dc.date.issued 2018
dc.identifier.citation Guarch, R., Lawrie, C. H., Larrinaga, G., Angulo, J. C., Pulido, R., & López, J. I. (2018). High levels of intratumor heterogeneity characterize the expression of epithelial-mesenchymal transition markers in high-grade clear cell renal cell carcinoma. Annals of Diagnostic Pathology, 34, 27-30. DOI: 10.1016/j.anndiagpath.2018.01.001 spa
dc.identifier.issn 1092-9134
dc.identifier.uri http://hdl.handle.net/11268/7374
dc.description.abstract Immunohistochemistry is a basic routine in establishing the diagnosis of many tumors. However, immunomarkers are often irregularly distributed across different regions of the same tumor, alternating positive and negative areas without any apparent cause. Full identification of this type of intratumor heterogeneity is crucial for patients since the expression of many markers is linked to the prognosis and/or treatment of some tumors. We have quantified this variability testing 406 tumor samples from eight clear cell renal cell carcinomas using four epithelial-mesenchymal transition markers (vimentin, ZEB-1, β-catenin, and E-cadherin) and two different sampling protocols. Routine sampling included an amount of 59 samples (average, 7.3 samples/case) and multisite tumor sampling did a total of 347 samples (average, 43.3 samples/case). High variability of immunostaining was detected with E-cadherin and ZEB-1 in all high-grade cases. Irregular patterns of expression were detected in all tumors including all histologically homogeneous low-grade tumors. Multisite tumor sampling protocol detected a significant decreased number of E-cadherin, β-catenin and ZEB-1 positive samples in high-grade tumors. We conclude that high levels of intratumor heterogeneity characterize the immunohistochemical expression of epithelial-mesenchymal transition markers in high-grade clear cell renal cell carcinomas. Multisite tumor sampling protocol outperforms routine sampling in detecting immunohistochemical intratumor heterogeneity. spa
dc.description.sponsorship SIN FINANCIACIÓN spa
dc.language.iso eng spa
dc.title High levels of intratumor heterogeneity characterize the expression of epithelial-mesenchymal transition markers in high-grade clear cell renal cell carcinoma spa
dc.type article spa
dc.description.impact 1.633 JCR (2017) Q3, 50/79 Pathology spa
dc.identifier.doi 10.1016/j.anndiagpath.2018.01.001
dc.rights.accessRights closedAccess spa
dc.subject.uem Células cancerosas spa
dc.subject.uem Riñones spa
dc.subject.uem Tumores spa
dc.subject.unesco Cáncer spa
dc.description.filiation UEM spa
dc.peerreviewed Si spa


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