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Shikonin Prevents Early Phase Inflammation Associated with Azoxymethane/Dextran Sulfate Sodium-Induced Colon Cancer and Induces Apoptosis in Human Colon Cancer Cells

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dc.contributor.author Andujar Pérez, Isabel
dc.contributor.author Giner, Rosa María
dc.contributor.author Ríos, José Luis
dc.contributor.author Recio, María Carmen
dc.date.accessioned 2018-09-29T21:31:05Z
dc.date.available 2018-09-29T21:31:05Z
dc.date.issued 2018
dc.identifier.citation Andújar, I., Martí-Rodrigo, A., Giner, R. M., Ríos, J. L., & Recio, M. C. (2018). Shikonin Prevents Early Phase Inflammation Associated with Azoxymethane/Dextran Sulfate Sodium-Induced Colon Cancer and Induces Apoptosis in Human Colon Cancer Cells. Planta Medica, 84(09/10), 674-683. DOI: 10.1055/a-0599-1145 spa
dc.identifier.issn 0032-0943
dc.identifier.uri http://hdl.handle.net/11268/7446
dc.description.abstract Shikonin is the main active principle in the root of Lithospermum erythrorhizon, widely used in traditional Chinese medicine for its anti-inflammatory and wound healing properties. Recent research highlights shikonin's antitumor properties and capacity to prevent acute ulcerative colitis. The aim of the present study was to evaluate the ability of shikonin to prevent, in vivo, the early phases of colorectal cancer development, with special focus on its cytotoxic mechanism in vitro. We employed the azoxymethane/dextran sulfate sodium model of colitis in Balb/C mice. Body weight and drinking were monitored throughout the experiment, and length of colon and lesions of the colon were recorded on termination of the experiment in all of the experimental groups. Colons underwent histological evaluation and biochemical analyses [myeloperoxidase activity assay, measurement of interleukin-6, evaluation of proinflammatory enzymes (cyclooxygenase-2 and inducible nitric oxide synthase), and nuclear factor-κB activation by Western blot]. Caco-2 cells were used to evaluate, in vitro, the effect of shikonin on proliferation, cytotoxicity, cell cycle, and apoptosis. Our results reveal that shikonin significantly protected the intestinal tissue of our animals by preventing the shortening of the colorectum and ulcer formation in a dose-dependent manner. Shikonin attenuated the expression of cyclooxygenase-2 and inducible nitric oxide synthase, and myeloperoxidase activity, and inhibited the production of interleukin-6 and activation of nuclear factor-κB. It induced Bcl-2 and inhibited caspase 3. In conclusion, shikonin acts as a chemopreventive agent in the azoxymethane/dextran sulfate sodium model through inhibition of the proinflammatory milieu generated during the disease, an important risk factor in cancer development. spa
dc.description.sponsorship Ministerio de Ciencia e Innovación (SAF2009-130593-C03-01) spa
dc.description.sponsorship Universitat de València (UV-INV-AE13-139455) spa
dc.language.iso eng spa
dc.title Shikonin Prevents Early Phase Inflammation Associated with Azoxymethane/Dextran Sulfate Sodium-Induced Colon Cancer and Induces Apoptosis in Human Colon Cancer Cells spa
dc.type article spa
dc.description.impact 2.494 JCR (2017) Q1, 6/27 Integrative and Complementary Medicine; Q2, 120/261 Pharmacology & Pharmacy spa
dc.identifier.doi 10.1055/a-0599-1145
dc.rights.accessRights closedAccess spa
dc.subject.uem Farmacología spa
dc.subject.unesco Farmacología spa
dc.description.filiation UEV spa
dc.relation.publisherversion https://www.thieme-connect.com/DOI/DOI?10.1055/a-0599 -1145 spa
dc.peerreviewed Si spa


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