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Effects of Hypoxic Living and Training on Gene Expression in an Obese Rat Model

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dc.contributor.author He, Zi-Hong spa
dc.contributor.author Feng, Lianshi spa
dc.contributor.author Zhang, Li spa
dc.contributor.author Lu, Yingli spa
dc.contributor.author Xu, Jianfang spa
dc.contributor.author Lucía Mulas, Alejandro spa
dc.date.accessioned 2013-11-27T17:26:32Z
dc.date.available 2013-11-27T17:26:32Z
dc.date.issued 2012 spa
dc.identifier.citation He, Z. H., Feng, L., Zhang, L., Lu, Y., Xu, J., & Lucía-Mulas, A. (2012). Effects of hypoxic living and training on gene expression in an obese rat model. Medicine and Science in Sports and Exercise, 44(6), 1013-1020. spa
dc.identifier.issn 01959131 spa
dc.identifier.uri http://hdl.handle.net/11268/815
dc.description.abstract The study's purpose was to determine in a rat obesity model the effects of normoxic training, sedentary hypoxic living, or hypoxic living plus training on the skeletal muscle messenger RNA (mRNA) levels of 14 genes involved in oxygen sensing (hypoxia-inducible factor 1α, vascular endothelial growth factor, myoglobin), glucose metabolism (glucose transporter 4, muscle phosphofructokinase), mitochondrial biogenesis (peroxisome proliferator-activated receptor γ coactivator 1-α, nuclear respiratory factor 1) and function (citrate synthase, mitochondrial-encoded cytochrome oxidase subunit 1), pH regulation (monocarboxylate transporter 1, carbonic anhydrase 3), and antioxidant defense (manganese superoxide dismutase, copper/zinc superoxide dismutase, glutathione S-transferase pi). One hundred thirty male 3-wk-old Sprague-Dawley rats were fed a high-fat diet (4100 kcal kg(-1)) for 3 months (all reaching a final weight >415 g) and then randomly assigned to the following groups (n = 10 per group): C (control, 2 d of sedentary living in normoxic conditions), TN1-TN4 (1-4 wk of normoxic treadmill training), SH1-SH4 (1-4 wk of sedentary hypoxic living (13.6% O2)), or TH1-TH4 (1-4 wk of hypoxic living (13.6% O2) + hypoxic treadmill training). Individual mRNA levels recorded for TN1-TN4, SH1-SH4, and TH1-TH4 were expressed relative to the mean obtained in C for each gene. Through a two-way ANOVA, a significant interaction (treatment × treatment duration) effect was detected on expression levels of mRNAs for hypoxia-inducible factor 1α, vascular endothelial growth factor, myoglobin, nuclear respiratory factor 1, citrate synthase, carbonic anhydrase 3, monocarboxylate transporter 1, copper/zinc superoxide dismutase, glutathione S-transferase pi, and manganese superoxide dismutase. Expression levels were overall highest when training and living under hypoxia, usually after 3 wk (TH3), i.e., 79%-99% higher than the lowest values (usually corresponding to TN2) and 15.5%-53.9% higher than the second highest values (usually TH4). Normoxic training elicited no greater response than hypoxic sedentary living. In conclusion, in our obese rat model, hypoxic living conditions, especially if accompanied by hypoxic exercise training, can lead to health-related molecular adaptations at the skeletal muscle level. spa
dc.language.iso eng spa
dc.subject.other Oxygen Sensing spa
dc.subject.other Fat Loss spa
dc.subject.other Mitochondria spa
dc.subject.other High-Fat Diet spa
dc.subject.other Human Skeletal-Muscle spa
dc.subject.other Messenger-Rna Expression spa
dc.subject.other Normobaric spa
dc.subject.other Hypoxia spa
dc.subject.other Lipid-Metabolism spa
dc.subject.other Exercise spa
dc.subject.other Enzymes spa
dc.subject.other Pgc-1-Alpha spa
dc.subject.other Hif-1-Alpha spa
dc.subject.other Sport Sciences spa
dc.title Effects of Hypoxic Living and Training on Gene Expression in an Obese Rat Model spa
dc.type article spa
dc.description.impact 4.475 JCR (2012) Q1, 4/84 Sport sciences spa
dc.identifier.doi 10.1249/MSS.0b013e3182442d82 spa
dc.rights.accessRights openAccess en
dc.subject.unesco Obesidad spa
dc.description.filiation UEM spa
dc.peerreviewed Si spa


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